Virology. 2005 Oct 25;341(2):171-8. Epub 2005 Aug 8.

RNA interference directed to CDK2 inhibits HIV-1 transcription.

Ammosova T, Berro R, Kashanchi F, Nekhai S.

Source

Center for Sickle Cell Disease, Howard University College of Medicine, NW, Washington, DC 20059, USA.

Abstract

We previously reported that cell cycle-dependent kinase 2 (CDK2) is required for human immunodeficiency virus-1 (HIV-1) Tat-dependent transcription in vitro. In the present study, CDK2-specific RNA interference in cultured HEK293T cells inhibited CDK2 expression and Tat-induced HIV-1 transcription from non-integrated HIV-1 promoter but not basal HIV-1 transcription or transcription from CMV or beta-actin promoters. Also, CDK2-specific RNA interference inhibited Tat-induced transcription from the integrated HIV-1 promoter in HeLa-CD4-LTR-beta-gal cells and potently blocked TNFalpha-induced HIV-1 viral replication in OM10.1 cells. CDK2-specific RNA interference did not have an effect on cell cycle progression, but it augmented TNFalpha-induced apoptosis of OM10.1 cells. Our results indicate that CDK2 participates in Tat-mediated HIV-1 transcription and may serve as a potential therapeutic target.

PMID:: 16085226; [PubMed - indexed for MEDLINE]

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