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Placenta. 2005 Sep-Oct;26(8-9):661-71. Epub 2004 Dec 8.

Monocyte chemotactic protein-1 expression is increased in human gestational tissues during term and preterm labor.

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  • 1Division of Reproductive Sciences, Department of Obstetrics and Gynecology, University of Utah Health Science Center, Salt Lake City, 84132, USA. mesplin@hsc.utah.edu

Abstract

OBJECTIVE:

Microarray analysis was used to characterize the labor-selective transcriptome of the human myometrium during labor. One highly up-regulated transcript, monocyte chemotactic protein-1 (MCP-1), was further characterized.

METHODS:

Expression of MCP-1 was evaluated in the myometrium, the placenta, the gestational membranes (GM) and the amniotic fluid (AF) by real time RT-PCR, Northern blot analysis and ELISA. The level of immunoreactive (IR) MCP-1 content of primary myometrial cultures treated with inflammatory cytokines was quantified by ELISA.

RESULTS:

Up-regulation of the myometrial MCP-1 transcript in term laboring patients was demonstrated by microarray and confirmed by real time (RT)-PCR and Northern blot analysis. Increased MCP-1 transcripts were demonstrated in GM during term labor. The IR content of myometrial MCP-1 was increased during term labor and in the AF from patients experiencing preterm delivery. Levels of IR MCP-1 increased in myometrial cultures in response to interleukin 1-beta.

CONCLUSION:

The expression of myometrial MCP-1 was significantly increased during term labor and was similarly increased in vitro in response to interleukin 1-beta, a pro-inflammatory substance known to play a role in preterm birth. The increased IR content of MCP-1 within the AF preceding preterm delivery may render this protein a useful predictor of preterm birth.

PMID:
16085045
[PubMed - indexed for MEDLINE]
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