Comparison of the genomes of M. hyopneumoniae strains J, 7448, and 232. Genome-specific regions (ICEH and R-M/T) and rearranged regions (regions 1 to 5) are indicated; CDSs located in these regions are described in Tables S1 to S3 in the supplemental material). CDSs that are not identical in two genomes or that have undergone rearrangements are represented by small black arrows. Mhy-J, M. hyopneumoniae strain J; Mhy-P, M. hyopneumoniae strain 7448; Mhy-232, M. hyopneumoniae strain 232. The axes show the scales, in kilobases.

Phylogenetic analysis of Mollicutes. (A) Phylogenetic tree of the Mollicutes based on the concatenated alignment of deduced amino acid sequences of 146 CDSs. Bootstrap support values (based on 100 replicates) are indicated near each node for neighbor joining with JTT distance, maximum parsimony, and TREE-PUZZLE maximum likelihood, respectively (the asterisk indicates an unconfirmed node). The main taxonomic groups within Mollicutes are shown on the right. The Mollicutes phytoplasma (“Candidatus Phytoplasma asteris” OY strain, AP006628) (Phy) from the Acholeplasma-Anaeroplasma-Phytoplasma clade, Mesoplasma florum (NC_006055) (Mfl) from the Entomoplasmatales, as well as Bacillus subtilis (NC000964) (Bsu) and Streptococcus pyogenes (NC002737) (Spy) were included as outgroups. (B) Phylogenetic tree with branch lengths proportional to divergence times based on a 146-CDS data set estimated with the r8s program. (C) Linearized tree based on 16S rRNA sequences estimated by NJ with Kimura's two-parameter distance estimate and 100 bootstrap replicates. Divergence times were estimated assuming a divergence rate of 1% per 50 million years. Differences between estimates were due to the different calibration methods. The abbreviations of the mycoplasma species are explained in Table 2, footnote b.

Similarity relationships between 12 Mollicute genomes. Connecting lines inside the circle denote BLAST matches between nodes, with each node representing a protein sequence. Connected nodes are shown in green. The color of the connecting line indicates the degree of similarity between nodes as follows: red, a score between 0.9 and 1.0; yellow, a score between 0.8 and 0.9; blue, a score between 0.7 to 0.8; and light gray, a score of <0.7 (PhyloGrapher software). The order of nodes, or protein sequences, in the graph was arranged according to the topology (shown outside the circle) grouping the 12 mollicute genomes. The abbreviations of the mycoplasma species are explained in Table 2, footnote b. Phy, Phytoplasma.

Schematic representation of C₁-THF metabolism in mycoplasmas. FolA, dihydrofolate reductase; FolD, methylenetetrahydrofolate dehydrogenase (NADP+); Fmt, methionyl-tRNA formyltransferase; FthS, formate-dihydrofolate ligase; GlyA, glycine hydroxymethyltransferase; ThyA, thymidylate synthase; ThyX, thymidylate synthase (FAD).