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Biochem J. 2005 Dec 1;392(Pt 2):353-62.

The basal proton conductance of mitochondria depends on adenine nucleotide translocase content.

Author information

  • 1Medical Research Council Dunn Human Nutrition Unit, Hills Road, Cambridge CB2 2XY, UK. martin.brand@mrc-dunn.cam.ac.uk

Abstract

The basal proton conductance of mitochondria causes mild uncoupling and may be an important contributor to metabolic rate. The molecular nature of the proton-conductance pathway is unknown. We show that the proton conductance of muscle mitochondria from mice in which isoform 1 of the adenine nucleotide translocase has been ablated is half that of wild-type controls. Overexpression of the adenine nucleotide translocase encoded by the stress-sensitive B gene in Drosophila mitochondria increases proton conductance, and underexpression decreases it, even when the carrier is fully inhibited using carboxyatractylate. We conclude that half to two-thirds of the basal proton conductance of mitochondria is catalysed by the adenine nucleotide carrier, independently of its ATP/ADP exchange or fatty-acid-dependent proton-leak functions.

PMID:
16076285
[PubMed - indexed for MEDLINE]
PMCID:
PMC1316271
Free PMC Article
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