Methods Mol Biol. 2005;304:221-8.

Plasma RNA viral load in HIV-1 group O infection by real-time PCR.

Source

Unité de Virologie, GRAM EA 2636, CHU Charles Nicolle, Rouen, France.

Abstract

HIV-1 group O strains are highly divergent, and are found mainly in central Africa. The clinical course of group O infection is identical to that of HIV-1 group M infection, with rapid onset of immunodeficiency. The important divergence of the HIV-1 group O strains lead to high limitations of the commercial tests. We describe here a method based on real-time polymerase chain reaction (PCR) to quantify plasma HIV-1 group O RNA. Primers amplify both HIV-1 group O and HIV-1 group M strains. Conversely, the probe is HIV-1 group O-specific. The standard used to quantify the clinical samples is an RNA solution resulting from the transcription of a plasmid including the amplified fragment of PCR. Our technique is capable of amplifying a wide range of HIV-1 group O strains belonging to the three current clades. This technique can be used to monitor HIV-1 group O viral load, which has previously been difficult.

PMID:: 16061979; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

RNA, Viral

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Springer

Medical

HIV/AIDS - MedlinePlus Health Information

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