The aim of this in vitro study was to measure key morphological features of pulp chambers from furcated maxillary bicuspid teeth. There were 107 random human maxillary bicuspid teeth used. Each bicuspid was radiographed using the Trophy RVG digital imaging system and a Belmont Acuray X-ray at 70 kVp. Measurements were made using the Digipan measuring mode of the Trophy system. Results were mean (mm): pulp chamber floor to furcation: 1.85 +/- 0.85; pulp chamber ceiling to furcation: 4.61 +/- 1.04; cusp to furcation: 11.55 +/- 1.12; cusp to pulp chamber ceiling: 6.94 +/- 0.70; pulp chamber height: 2.76 +/- 0.97. The measurements showing the lowest percentage variance were: cusp to furcation (9.70%) and cusp to pulp chamber ceiling (10.09%). The only measurement that was statistically the same across maxillary molars, mandibular molars and bicuspids was measurement "B," pulp chamber ceiling to furcation. The critical distance from cusp tip to pulp chamber ceiling in bicuspids was approximately 7.00 mm.