Abstract
Resistant C57BL/6 mice infected in the lungs with Mycobacterium tuberculosis and then therapeutically vaccinated with Mycobacterium leprae-derived hsp65 DNA develop severe granulomatous pneumonia and tissue damage. Analysis of cells accumulating in the lungs of these animals revealed substantial increases in T cells secreting tumor necrosis factor alpha and CD8 cells staining positive for granzyme B. Stimulation of lung cells ex vivo revealed very high levels of interleukin-10, some of which was produced by B-1 B cells. This was probably an anti-inflammatory response, since lung pathology was dramatically worsened in B-cell gene-disrupted mice.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Bacterial Proteins / genetics*
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Chaperonin 60
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Chaperonins / genetics*
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DNA / therapeutic use*
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Female
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Immunohistochemistry
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Lung / immunology
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Lung / microbiology
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Lung / pathology
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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Mycobacterium leprae / genetics
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Mycobacterium leprae / immunology
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Mycobacterium tuberculosis / immunology
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Tuberculosis, Pulmonary / drug therapy*
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Tuberculosis, Pulmonary / immunology
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Tuberculosis, Pulmonary / microbiology
Substances
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Bacterial Proteins
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Chaperonin 60
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heat-shock protein 65, Mycobacterium
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DNA
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Chaperonins