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Science. 2005 Aug 19;309(5738):1248-51. Epub 2005 Jul 14.

Genome-wide RNAi screen for host factors required for intracellular bacterial infection.

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  • 1Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA. herve.agaisse@yale.edu

Abstract

Most studies of host-pathogen interactions have focused on pathogen-specific virulence determinants. Here, we report a genome-wide RNA interference screen to identify host factors required for intracellular bacterial pathogenesis. Using Drosophila cells and the cytosolic pathogen Listeria monocytogenes, we identified 305 double-stranded RNAs targeting a wide range of cellular functions that altered L. monocytogenes infection. Comparison to a similar screen with Mycobacterium fortuitum, a vacuolar pathogen, identified host factors that may play a general role in intracellular pathogenesis and factors that specifically affect access to the cytosol by L. monocytogenes.

PMID:
16020693
[PubMed - indexed for MEDLINE]
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