Abstract
Most studies of host-pathogen interactions have focused on pathogen-specific virulence determinants. Here, we report a genome-wide RNA interference screen to identify host factors required for intracellular bacterial pathogenesis. Using Drosophila cells and the cytosolic pathogen Listeria monocytogenes, we identified 305 double-stranded RNAs targeting a wide range of cellular functions that altered L. monocytogenes infection. Comparison to a similar screen with Mycobacterium fortuitum, a vacuolar pathogen, identified host factors that may play a general role in intracellular pathogenesis and factors that specifically affect access to the cytosol by L. monocytogenes.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Cell Cycle
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Cell Line
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Cytoskeleton / metabolism
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Cytosol / microbiology
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Drosophila Proteins / genetics
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Drosophila Proteins / physiology*
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Drosophila melanogaster / cytology
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Drosophila melanogaster / genetics*
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Drosophila melanogaster / metabolism
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Drosophila melanogaster / microbiology*
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Genes, Insect*
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Genome
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Green Fluorescent Proteins / metabolism
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Listeria monocytogenes / growth & development
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Listeria monocytogenes / pathogenicity
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Listeria monocytogenes / physiology*
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Macrophages / microbiology*
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Macrophages / physiology
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Mycobacterium fortuitum / growth & development
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Mycobacterium fortuitum / pathogenicity
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Mycobacterium fortuitum / physiology
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Phenotype
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RNA Interference*
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RNA Processing, Post-Transcriptional
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RNA, Double-Stranded / pharmacology
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Signal Transduction / genetics
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Vacuoles / microbiology
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Vesicular Transport Proteins / genetics
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Vesicular Transport Proteins / metabolism
Substances
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Drosophila Proteins
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RNA, Double-Stranded
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Vesicular Transport Proteins
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Green Fluorescent Proteins