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1: J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Aug 5;822(1-2):238-43.Click here to read Links

High-performance liquid chromatography-mass spectrometry method for determination of anagrelide in human plasma.

Zhu Z, Gonthier R, Neirinck L.

Pharmascience, 6111 Royalmount Ave. Suite 100, Montreal, Que., Canada. zzhu@pharmascience.com

This paper describes a simple, fast and sensitive liquid chromatography-mass spectrometry method for quantification of an anti-thrombocythemic agent, anagrelide in human plasma. The samples were subjected to a liquid-liquid extraction after addition of a buffer and an internal standard. Chromatography was performed on an Inertsil ODS2 column and the extract was injected onto a HPLC system coupled with mass spectrometric detection. Linear responses for standards were observed from 50 to 7500 pg/ml. The accuracy of intra-assay and inter-assay were in the ranges 4.3-4.4% and 4.8-5.6%, respectively. The method is simple and reproducible with a run time of less than 2 min.

PMID: 16005692 [PubMed - indexed for MEDLINE]

Patient Drug Information

  • Anagrelide (Agrylin® )

    Anagrelide is used to decrease the number of platelets (a type of blood cell that is needed to control bleeding) in the blood of patients who have a myeloproliferative disorder (condition in which the body makes too many...

  • Source: AHFS Consumer Medication Information