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Appl Opt. 2005 Jul 1;44(19):3977-84.

Applications of short-coherence digital holography in microscopy.

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  • 1Departament de Ciències Experimentals, Universitat Jaume I, 12071 Castelló, Spain.


We present an optical system based on short-coherence digital holography suitable for the imaging of three-dimensional microscopic objects. The short temporal coherence properties of the light source allow optical sectioning of the sample. Proper reconstruction of different layers within biological samples is possible up to a depth of a few hundred micrometers, but multiple scattering and inhomogeneities in the refractive index reduce the imaging quality for deeper layers. We have studied the possibility of numerically correcting sample-induced aberrations, and we now propose a method of improving image quality. Numerical simulations and preliminary experimental results show that compensation of these aberrations is possible to some extent.

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