Changes in neuronal activity have been shown to be accompanied by alteration in regional cerebral blood flow . In the present study, laser speckle imaging (LSI) was employed to measure stimulus-evoked neuronal activities in rat barrel cortex. The spatiotemporal characteristics of hemodynamic response to mechanical stimuli from 1 to 3 Hz were examined. Time to peak amplitude reduced from 4.5 to 3.5 s with increasing frequencies. Spatially, the response was confined to a small circular region at the beginning and then spread out asymmetrically to the surrounding regions. The maximal area of activation ranged from 2.2 to 3.5 mm2, while the time to reach maximal area occurred between 5.5 and 6 s. Moreover, there was a high correlation between LSI and laser-Doppler flowmetry in terms of peak response magnitude and the time to reach peak. These two values were linearly dependent on stimulus frequency whereas area of activation and time to maximal area appeared to be independent of this parameter. LSI's high sensitivity, low cost of the equipment, and size and complexity make this a suitable technique for fundamental neurophysiological investigations.