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    Appl Environ Microbiol. 2005 Jul;71(7):3761-9.

    Enhanced trehalose production improves growth of Escherichia coli under osmotic stress.

    Purvis JE, Yomano LP, Ingram LO.

    Source

    Dept. of Microbiology and Cell Science, Bldg. 981 Museum Road, IFAS, P.O. Box 110700, University of Florida, Gainesville, FL 32611, USA.

    Abstract

    The biosynthesis of trehalose has been previously shown to serve as an important osmoprotectant and stress protectant in Escherichia coli. Our results indicate that overproduction of trehalose (integrated lacI-Ptac-otsBA) above the level produced by the native regulatory system can be used to increase the growth of E. coli in M9-2% glucose medium at 37 degrees C to 41 degrees C and to increase growth at 37 degrees C in the presence of a variety of osmotic-stress agents (hexose sugars, inorganic salts, and pyruvate). Smaller improvements were noted with xylose and some fermentation products (ethanol and pyruvate). Based on these results, overproduction of trehalose may be a useful trait to include in biocatalysts engineered for commodity chemicals.

    PMID:
    16000787
    [PubMed - indexed for MEDLINE]
    PMCID: PMC1168978
    Free PMC Article

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