Microb Cell Fact. 2005 Jul 5;4:21.

Structural genomics of human proteins--target selection and generation of a public catalogue of expression clones.

Büssow K, Scheich C, Sievert V, Harttig U, Schultz J, Simon B, Bork P, Lehrach H, Heinemann U.

Source

Protein Structure Factory, Heubnerweg 6, 14059 Berlin, Germany. buessow@molgen.mpg.de

Abstract

BACKGROUND: The availability of suitable recombinant protein is still a major bottleneck in protein structure analysis. The Protein Structure Factory, part of the international structural genomics initiative, targets human proteins for structure determination. It has implemented high throughput procedures for all steps from cloning to structure calculation. This article describes the selection of human target proteins for structure analysis, our high throughput cloning strategy, and the expression of human proteins in Escherichia coli host cells. RESULTS AND CONCLUSION: Protein expression and sequence data of 1414 E. coli expression clones representing 537 different proteins are presented. 139 human proteins (18%) could be expressed and purified in soluble form and with the expected size. All E. coli expression clones are publicly available to facilitate further functional characterisation of this set of human proteins.

PMID:: 15998469; [PubMed]
PMCID:: PMC1250228

Free PMC Article

Images from this publication.See all images (3) Free text

LinkOut - more resources

Full Text Sources

Supplemental Content

Save items

Related citations in PubMed

Domain selection combined with improved cloning strategy for high throughput expression of higher eukaryotic proteins. [BMC Biotechnol. 2007]
Domain selection combined with improved cloning strategy for high throughput expression of higher eukaryotic proteins.
Chen Y, Qiu S, Luan CH, Luo M. BMC Biotechnol. 2007 Jul 30; 7:45. Epub 2007 Jul 30.
Parallel cloning, expression, purification and crystallization of human proteins for structural genomics. [Acta Crystallogr D Biol Crysta...]
Parallel cloning, expression, purification and crystallization of human proteins for structural genomics.
Ding HT, Ren H, Chen Q, Fang G, Li LF, Li R, Wang Z, Jia XY, Liang YH, Hu MH, et al. Acta Crystallogr D Biol Crystallogr. 2002 Dec; 58(Pt 12):2102-8. Epub 2002 Nov 23.
Fast identification of folded human protein domains expressed in E. coli suitable for structural analysis. [BMC Struct Biol. 2004]
Fast identification of folded human protein domains expressed in E. coli suitable for structural analysis.
Scheich C, Leitner D, Sievert V, Leidert M, Schlegel B, Simon B, Letunic I, Büssow K, Diehl A. BMC Struct Biol. 2004 Mar 8; 4:4. Epub 2004 Mar 8.
Review Protein expression systems for structural genomics and proteomics. [Curr Opin Chem Biol. 2003]
Review Protein expression systems for structural genomics and proteomics.
Yokoyama S. Curr Opin Chem Biol. 2003 Feb; 7(1):39-43.
Review Target selection for structural genomics: an overview. [Methods Mol Biol. 2008]
Review Target selection for structural genomics: an overview.
Marsden RL, Orengo CA. Methods Mol Biol. 2008; 426:3-25.

See reviews... See all...

Cited by 6 PubMed Central articles

Co-crystal structures of PKG Iβ (92-227) with cGMP and cAMP reveal the molecular details of cyclic-nucleotide binding. [PLoS One. 2011]
Co-crystal structures of PKG Iβ (92-227) with cGMP and cAMP reveal the molecular details of cyclic-nucleotide binding.
Kim JJ, Casteel DE, Huang G, Kwon TH, Ren RK, Zwart P, Headd JJ, Brown NG, Chow DC, Palzkill T, et al. PLoS One. 2011 Apr 19; 6(4):e18413. Epub 2011 Apr 19.
The scientific impact of microbial cell factories. [Microb Cell Fact. 2008]
The scientific impact of microbial cell factories.
De Felice M, Mattanovich D, Papagianni M, Wegrzyn G, Villaverde A. Microb Cell Fact. 2008 Dec 1; 7:33. Epub 2008 Dec 1.
Review Protein production and purification. [Nat Methods. 2008]
Review Protein production and purification.
Structural Genomics Consortium, China Structural Genomics Consortium, Northeast Structural Genomics Consortium, Gräslund S, Nordlund P, Weigelt J, Hallberg BM, Bray J, Gileadi O, Knapp S, et al. Nat Methods. 2008 Feb; 5(2):135-46.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
References for this PMC Article
Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
Free in PMC
Free full text articles in PMC
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Structural genomics of human proteins--target selection and generation of a publ...
Structural genomics of human proteins--target selection and generation of a public catalogue of expression clones.
Microb Cell Fact. 2005 Jul 5 ;4:21.

PubMed
Mast fruiting and seedling survival of the ectomycorrhizal, monodominant Dicymbe...
Mast fruiting and seedling survival of the ectomycorrhizal, monodominant Dicymbe corymbosa (Caesalpiniaceae) in Guyana.
New Phytol. 2005 Aug ;167(2):543-56.

PubMed
A triple mutation in the second transmembrane domain of mouse dopamine transport...
A triple mutation in the second transmembrane domain of mouse dopamine transporter markedly decreases sensitivity to cocaine and methylphenidate.
J Neurochem. 2005 Jul ;94(2):352-9.

PubMed
Amyloid beta peptide 1-40 enhances the action of Toll-like receptor-2 and -4 ago...
Amyloid beta peptide 1-40 enhances the action of Toll-like receptor-2 and -4 agonists but antagonizes Toll-like receptor-9-induced inflammation in primary mouse microglial cell cultures.
J Neurochem. 2005 Jul ;94(2):289-98.

PubMed
Similar medication compliance and control of dyslipidemia with simvastatin or at...
Similar medication compliance and control of dyslipidemia with simvastatin or atorvastatin in a staff-model HMO medical clinic.
J Manag Care Pharm. 2005 Jul-Aug ;11(6):499-504.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

Structural genomics of human proteins--target selection and generation of a public catalogue of expression clones.

Source

Abstract

LinkOut - more resources

Full Text Sources

Supplemental Content

Save items

Related citations in PubMed

Cited by 6 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information