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Genome Biol. 2005;6(7):R63. Epub 2005 Jun 10.

Genomic analysis of heat-shock factor targets in Drosophila.

Author information

  • 1Department of Anatomy, University of Cambridge, Downing Street, Cambridge, CB2 3EH, UK. ib107@cam.ac.uk

Abstract

We have used a chromatin immunoprecipitation-microarray (ChIP-array) approach to investigate the in vivo targets of heat-shock factor (Hsf) in Drosophila embryos. We show that this method identifies Hsf target sites with high fidelity and resolution. Using cDNA arrays in a genomic search for Hsf targets, we identified 141 genes with highly significant ChIP enrichment. This study firmly establishes the potential of ChIP-array for whole-genome transcription factor target mapping in vivo using intact whole organisms.

PMID:
15998452
[PubMed - indexed for MEDLINE]
PMCID:
PMC1175994
Free PMC Article

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