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1: Genome Biol. 2005;6(7):R63. Epub 2005 Jun 10.Click here to read Click here to read Links

Genomic analysis of heat-shock factor targets in Drosophila.

Birch-Machin I, Gao S, Huen D, McGirr R, White RA, Russell S.

Department of Anatomy, University of Cambridge, Downing Street, Cambridge, CB2 3EH, UK. ib107@cam.ac.uk

We have used a chromatin immunoprecipitation-microarray (ChIP-array) approach to investigate the in vivo targets of heat-shock factor (Hsf) in Drosophila embryos. We show that this method identifies Hsf target sites with high fidelity and resolution. Using cDNA arrays in a genomic search for Hsf targets, we identified 141 genes with highly significant ChIP enrichment. This study firmly establishes the potential of ChIP-array for whole-genome transcription factor target mapping in vivo using intact whole organisms.

PMID: 15998452 [PubMed - indexed for MEDLINE]

PMCID: PMC1175994