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Genome Biol. 2005;6(7):R62. Epub 2005 Jul 1.

Validation and refinement of gene-regulatory pathways on a network of physical interactions.

Author information

  • 1Center for Biomolecular Science and Engineering, Baskin School of Engineering, University of California, Santa Cruz, CA 95064, USA. chyeang@soe.ucsc.edu

Abstract

As genome-scale measurements lead to increasingly complex models of gene regulation, systematic approaches are needed to validate and refine these models. Towards this goal, we describe an automated procedure for prioritizing genetic perturbations in order to discriminate optimally between alternative models of a gene-regulatory network. Using this procedure, we evaluate 38 candidate regulatory networks in yeast and perform four high-priority gene knockout experiments. The refined networks support previously unknown regulatory mechanisms downstream of SOK2 and SWI4.

PMID:
15998451
[PubMed - indexed for MEDLINE]
PMCID:
PMC1175993
Free PMC Article

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