Transcript copy number estimation using a mouse wh...[Genome Biol. 2005]

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1: Genome Biol. 2005;6(7):R61. Epub 2005 Jun 30. Links

Transcript copy number estimation using a mouse whole-genome oligonucleotide microarray.

Carter MG, Sharov AA, VanBuren V, Dudekula DB, Carmack CE, Nelson C, Ko MS.

Laboratory of Genetics, National Institute on Aging, National Institutes of Health, 333 Cassell Drive, Baltimore, MD 21224, USA. cartermar@mail.nih.gov

The ability to quantitatively measure the expression of all genes in a given tissue or cell with a single assay is an exciting promise of gene-expression profiling technology. An in situ-synthesized 60-mer oligonucleotide microarray designed to detect transcripts from all mouse genes was validated, as well as a set of exogenous RNA controls derived from the yeast genome (made freely available without restriction), which allow quantitative estimation of absolute endogenous transcript abundance.

PMID: 15998450 [PubMed - indexed for MEDLINE]

PMCID: PMC1175992

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Cited by 18 PubMed Central articles

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Cited by 18 PubMed Central articles

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