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PLoS Biol. 2005 Aug;3(8):e248. Epub 2005 Jul 5.

The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite.

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  • 1Structural and Genomic Information Laboratory, UPR 2589, IBSM, CNRS, Marseille Cedex, France. Hiroyuki.Ogata@igs.cnrs-mrs.fr

Abstract

We sequenced the genome of Rickettsia felis, a flea-associated obligate intracellular alpha-proteobacterium causing spotted fever in humans. Besides a circular chromosome of 1,485,148 bp, R. felis exhibits the first putative conjugative plasmid identified among obligate intracellular bacteria. This plasmid is found in a short (39,263 bp) and a long (62,829 bp) form. R. felis contrasts with previously sequenced Rickettsia in terms of many other features, including a number of transposases, several chromosomal toxin-antitoxin genes, many more spoT genes, and a very large number of ankyrin- and tetratricopeptide-motif-containing genes. Host-invasion-related genes for patatin and RickA were found. Several phenotypes predicted from genome analysis were experimentally tested: conjugative pili and mating were observed, as well as beta-lactamase activity, actin-polymerization-driven mobility, and hemolytic properties. Our study demonstrates that complete genome sequencing is the fastest approach to reveal phenotypic characters of recently cultured obligate intracellular bacteria.

PMID:
15984913
[PubMed - indexed for MEDLINE]
PMCID:
PMC1166351
Free PMC Article

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