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Obes Surg. 2005 Jun-Jul;15(6):758-65.

Obesity-related differential gene expression in the visceral adipose tissue.

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  • 1Center for the Study of Genomics in Liver Diseases, Molecular and Microbiology Department, George Mason University, VA, USA.

Abstract

BACKGROUND:

This study investigates the expression patterns in human adipose tissue, and identifies genes that may be involved in the abnormal energy homeostasis.

METHODS:

Subjects were prospectively recruited from morbidly obese patients undergoing bariatric surgery and from non-obese organ donors. Extensive clinical data and visceral fat specimens were obtained from each subject at the time of surgery. A group of 50 obese patients and 9 non-obese controls were selected for further study. Two custom two-color cDNA microarrays were produced with 40,173 human individual cDNA clones. Microarray experiments were performed for each sample, and a selected group of gene expression values were confirmed with real-time RT-PCR.

RESULTS:

A comparison of gene expression profiles from obese and non-obese patients identified 1,208 genes with statistically significant differential expression between the 2 groups. Most prominent among these genes are multiple glycolysis enzyme encoding genes; others are involved in oxysterol biosynthesis and signaling, or are ATP-binding transporters and solute carriers.

CONCLUSION:

Differential gene expression in the adipose tissue of morbidly obese patients includes genes related to lipid and glucose metabolism, membrane transport, and genes promoting the cell cycle. These findings are a first step toward clarifying the molecular pathogenesis of obesity and identifying potential targets for therapeutic intervention.

[PubMed - indexed for MEDLINE]
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