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Zhonghua Gan Zang Bing Za Zhi. 2005 Jun;13(6):428-31.

[Relationship between DLC-1 expressions and metastasis in hepatocellular carcinoma].

[Article in Chinese]

Author information

  • 1Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai 200032, China.

Abstract

OBJECTIVES:

To study the relationship between the expression level of DLC-1 mRNA (located in 8p) and the invasion/metastasis of human hepatocellular carcinoma (HCC).

METHODS:

Fifty-one surgical specimens of human HCC were divided into high-invasive and low invasive groups according to their clinicopathological features. DLC-1 mRNA expression was studied in the 51 HCC specimens as well as 5 different metastasis potential cell lines using real-time quantitative PCR (RQ-PCR).

RESULTS:

The expression level of DLC-1 mRNA in HCC specimens with high invasiveness was significantly lower than that with low invasiveness (P < 0.05). The expression levels of DLC-1 mRNA were significantly different between non-metastatic (Hep3B and HepG2) and metastatic (MHCC97-H, MHCC97-L and HCCLM3) cell lines (P < 0.05). From MHCC97-L to HCCLM3, with an increase of invasiveness and metastatic potentials, the expression level of DLC-1 decreased correspondingly, and its expression level in HCCLM3 was significantly lower than that in MHCC97-L (P < 0.01).

CONCLUSION:

The expression of DLC-1 mRNA may play an important role in inhibiting the invasiveness and metastasis of HCC.

PMID:
15975276
[PubMed - indexed for MEDLINE]
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