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Genome Biol. 2005;6(6):R54. Epub 2005 May 11.

Single-feature polymorphism discovery in the barley transcriptome.

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  • 1Scottish Crop Research Institute, Genome Dynamics, Invergowrie, Dundee, DD2 5DA, Scotland, UK.

Abstract

A probe-level model for analysis of GeneChip gene-expression data is presented which identified more than 10,000 single-feature polymorphisms (SFP) between two barley genotypes. The method has good sensitivity, as 67% of known single-nucleotide polymorphisms (SNP) were called as SFPs. This method is applicable to all oligonucleotide microarray data, accounts for SNP effects in gene-expression data and represents an efficient and versatile approach for highly parallel marker identification in large genomes.

PMID:
15960806
[PubMed - indexed for MEDLINE]
PMCID:
PMC1175974
Free PMC Article

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