Two percent agarose gel electrophoresis of an intergenic spacer region PCR amplification (primers 321s and 983as) of Bartonella cultures in BAPGM (lanes 2 to 9, at 7 days postinoculation; lanes 10 to 16, 5 days postinoculation). Lane 1, 1-kbp DNA ladder; lane 2, B. elizabethae (717 bp); lane 3, B. grahamii (658 bp); lane 4, B. quintana Fuller (564 bp); lanes 5 and 12, B. vinsonii (berkhoffii) (706 bp); lane 6, B. henselae Houston-1 (648 bp); lane 7, B. clarridgeiae (636 bp); lane 8, B. doshiae (550 bp); lanes 9 and 16, BAPGM as a negative control; lane 10, B. henselae; lane 11, B. quintana; lane 13, B. henselae and B. quintana coculture; lane 14, B. henselae and B. vinsonii (berkhoffii) coculture; lane 15, B. quintana and B. vinsonii (berkhoffii) coculture.