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Opt Lett. 2005 May 15;30(10):1162-4.

Spectral-domain phase microscopy.

Author information

  • 1Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA. mac32@duke.edu

Abstract

Broadband interferometry is an attractive technique for the detection of cellular motions because it provides depth-resolved phase information via coherence gating. We present a phase-sensitive technique called spectral-domain phase microscopy (SDPM). SDPM is a functional extension of spectral-domain optical coherence tomography that allows for the detection of nanometer-scale motions in living cells. The sensitivity of the technique is demonstrated, and its calibration is verified. A shot-noise limit to the displacement sensitivity of this technique is derived. Measurement of cellular dynamics was performed on spontaneously beating cardiomyocytes isolated from chick embryos.

PMID:
15945141
[PubMed - indexed for MEDLINE]
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