Display Settings:

Format

Send to:

Choose Destination
We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
    Microbiology. 2005 Jun;151(Pt 6):1769-78.

    The Bacillus subtilis YdfHI two-component system regulates the transcription of ydfJ, a member of the RND superfamily.

    Source

    Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Ueda-shi, Nagano 386-8567, Japan.

    Abstract

    The ydfHI genes encode a sensor kinase and a response regulator forming a two-component system. ydfJ is located downstream of ydfHI, and belongs to the RND (resistance-nodulation-cell division) superfamily, which is present in most major organisms. Four genes (secDF, yerP, ydfJ and ydgH) in Bacillus subtilis belong to this family. This study revealed that the YdfHI two-component system regulates ydfJ transcription. A gel shift assay using histidine-tagged YdfI (h-YdfI) showed that it directly binds to the ydfJ promoter region. Moreover, DNase I footprinting analysis revealed a tandem repeat sequence consisting of two conserved 12-mer sequences (GCCCRAAYGTAC) within the h-YdfI-binding site.

    PMID:
    15941986
    [PubMed - indexed for MEDLINE]
    Free full text

      Supplemental Content

      Icon for HighWire

      Save items

      Recent activity

      Your browsing activity is empty.

      Activity recording is turned off.

      Turn recording back on

      See more...
      Write to the Help Desk