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Cell Immunol. 2004 Sep-Oct;231(1-2):8-13. Epub 2004 Dec 19.

Bryostatin-1 in combination with calcium ionophore promotes the maturation of human umbilical cord-blood monocyte-derived dendritic cells capable of activating neonatal alloreactive T cells.

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  • 1Department of Microbiology and Immunology, Medical College of Virginia Campus, Virginia Commonwealth University, Richmond, VA 23298-0678, USA.

Abstract

We have investigated the effect of bryostatin-1 (Bryo-1) and calcium ionophore (CI) on maturation and functions of DCs generated from adherent cells of cord blood cultured with GM-CSF plus IL-4 (CB-DCs). The CB-DCs treated with Bryo-1+CI exhibited morphologic characteristics of mature DCs, expressed increased levels of CD1a, CD80, CD83, CD86, and MHC class II as well as enhanced ability to induce proliferation of alloreactive T cells isolated from cord blood and IFN-gamma production. Treatment of CB-DCs with TNF-alpha or PMA+CI was less effective. Thus, Bryo-1+CI promotes maturation of CB-DCs and therefore could be used to enhance the neonatal immune response.

PMID:
15919364
[PubMed - indexed for MEDLINE]
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