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Biochem Biophys Res Commun. 1992 May 15;184(3):1311-6.

Platelet-derived endothelial cell growth factor has thymidine phosphorylase activity.

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  • 1Ludwig Institute for Cancer Research, Biomedical Center, Uppsala, Sweden.

Abstract

Platelet-derived endothelial cell growth factor (PD-ECGF), a protein which stimulates angiogenesis in vivo, is shown to have a 39.2% amino acid sequence similarity over a 439 amino acid region with the thymidine phosphorylase of Escherichia coli (E. coli). Using recombinant human PD-ECGF, we show that PD-ECGF has thymidine phosphorylase activity. Analysis by gel chromatography revealed that recombinant human PD-ECGF occurs as a 90 kDa homodimer, similar to other thymidine phosphorylases. In addition to a possible effect on DNA synthesis, PD-ECGF was shown to affect [3H]thymidine assays in a manner which is not related to cell proliferation. The in vitro and in vivo effects of PD-ECGF may thus occur by an indirect mechanism through its enzymatic activity.

PMID:
1590793
[PubMed - indexed for MEDLINE]
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