Comparison of ensemble and single molecule order parameter measurements. The solid triangle and square show
and
respectively, determined according to Eqs. 2 and 3 from the distribution of 148 single molecule measurements of
β shown in Fig. 4. The product of these values (see Eq. 4) is shown as the solid circle. The open circle indicates the order parameters determined by summing all of the single molecule intensities and then analyzing these sums as though they were from a densely labeled filament. Dark and shaded diamonds indicate the order parameters determined for densely labeled horizontally aligned (along the
x axis) and vertically aligned (along the
y axis) filaments, respectively. All physical angular distributions fall within the area bounded by the solid lines.