Structure. 2005 May;13(5):703-12.

A coiled-coil structure of the alphaIIbbeta3 integrin transmembrane and cytoplasmic domains in its resting state.

Source

Department of Biological Chemistry, The Weizmann Institute of Science, Herzl St. 1, 76100 Rehovot, Israel. kay.gottschalk@physik.uni-muenchen.de

Abstract

One of the hallmark features of the integrin receptors is the ability to transmit signals bidirectionally through the cell membrane. The transmembrane integrin domains are pivotal to the signaling events. An understanding of the signaling mechanism requires structural information. Here, we report a structural model of the transmembrane and part of the cytosolic domains of the alphaIIbbeta3 integrin in its resting state. The model was obtained computationally by a restrained conformational search of helix-helix interactions. It agrees with one published NMR structure of the cytoplasmic complex and can put many experimental findings on structural grounds. According to our model, integrins form an intricately designed coiled-coil structure in the resting state. The conserved Glycophorin A (GpA)-like sequence motif of the alpha, but not the beta, subunit, is in the interface of this model. Based on our calculations and other data, a signaling mechanism that involves a transient GpA-like structure is proposed.

Comment in

Structure. 2005 May;13(5):683-4.

PMID:: 15893661; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

Labome Researcher Resource - ExactAntigen/Labome

Supplemental Content

Save items

Related citations in PubMed

The structure of a receptor with two associating transmembrane domains on the cell surface: integrin alphaIIbbeta3. [Mol Cell. 2009]
The structure of a receptor with two associating transmembrane domains on the cell surface: integrin alphaIIbbeta3.
Zhu J, Luo BH, Barth P, Schonbrun J, Baker D, Springer TA. Mol Cell. 2009 Apr 24; 34(2):234-49.
Review Structures of integrin domains and concerted conformational changes in the bidirectional signaling mechanism of alphaIIbbeta3. [Exp Biol Med (Maywood). 2004]
Review Structures of integrin domains and concerted conformational changes in the bidirectional signaling mechanism of alphaIIbbeta3.
Calvete JJ. Exp Biol Med (Maywood). 2004 Sep; 229(8):732-44.
Structure of an integrin alphaIIb beta3 transmembrane-cytoplasmic heterocomplex provides insight into integrin activation. [Proc Natl Acad Sci U S A. 2009]
Structure of an integrin alphaIIb beta3 transmembrane-cytoplasmic heterocomplex provides insight into integrin activation.
Yang J, Ma YQ, Page RC, Misra S, Plow EF, Qin J. Proc Natl Acad Sci U S A. 2009 Oct 20; 106(42):17729-34. Epub 2009 Oct 1.
Transmembrane domain helix packing stabilizes integrin alphaIIbbeta3 in the low affinity state. [J Biol Chem. 2005]
Transmembrane domain helix packing stabilizes integrin alphaIIbbeta3 in the low affinity state.
Partridge AW, Liu S, Kim S, Bowie JU, Ginsberg MH. J Biol Chem. 2005 Feb 25; 280(8):7294-300. Epub 2004 Dec 10.
Review Structural basis of integrin transmembrane activation. [J Cell Biochem. 2010]
Review Structural basis of integrin transmembrane activation.
Wang W, Luo BH. J Cell Biochem. 2010 Feb 15; 109(3):447-52.

See reviews... See all...

Cited by 12 PubMed Central articles

A helix heterodimer in a lipid bilayer: prediction of the structure of an integrin transmembrane domain via multiscale simulations. [Structure. 2011]
A helix heterodimer in a lipid bilayer: prediction of the structure of an integrin transmembrane domain via multiscale simulations.
Kalli AC, Hall BA, Campbell ID, Sansom MS. Structure. 2011 Oct 12; 19(10):1477-84.
Consensus motif for integrin transmembrane helix association. [Proc Natl Acad Sci U S A. 2010]
Consensus motif for integrin transmembrane helix association.
Berger BW, Kulp DW, Span LM, DeGrado JL, Billings PC, Senes A, Bennett JS, DeGrado WF. Proc Natl Acad Sci U S A. 2010 Jan 12; 107(2):703-8. Epub 2009 Dec 18.
The structure of a receptor with two associating transmembrane domains on the cell surface: integrin alphaIIbbeta3. [Mol Cell. 2009]
The structure of a receptor with two associating transmembrane domains on the cell surface: integrin alphaIIbbeta3.
Zhu J, Luo BH, Barth P, Schonbrun J, Baker D, Springer TA. Mol Cell. 2009 Apr 24; 34(2):234-49.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

A coiled-coil structure of the alphaIIbbeta3 integrin transmembrane and cytoplas...
A coiled-coil structure of the alphaIIbbeta3 integrin transmembrane and cytoplasmic domains in its resting state.
Structure. 2005 May ;13(5):703-12.

PubMed
NF-(kappa)B mediates amyloid beta peptide-stimulated activity of the human apoli...
NF-(kappa)B mediates amyloid beta peptide-stimulated activity of the human apolipoprotein E gene promoter in human astroglial cells.
Brain Res Mol Brain Res. 2005 May 20 ;136(1-2):177-88.

PubMed
Evaluation, prognosis, and medical treatment considerations of metastatic bone t...
Evaluation, prognosis, and medical treatment considerations of metastatic bone tumors.
Orthopedics. 1992 May ;15(5):589-96.

PubMed
Behavioural, ventilatory and respiratory responses of epigean and hypogean crust...
Behavioural, ventilatory and respiratory responses of epigean and hypogean crustaceans to different temperatures.
Comp Biochem Physiol A Mol Integr Physiol. 2005 May ;141(1):1-7.

PubMed
Poor antisaccade performance in schizophrenia: an inhibition deficit?
Poor antisaccade performance in schizophrenia: an inhibition deficit?
Psychiatry Res. 2005 May 15 ;135(1):1-10.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: