Biochim Biophys Acta. 2005 Jun 30;1729(2):135-40. Epub 2005 Apr 25.

Identification of a structural element that is essential for two functions of transcription factor NusG.

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Department of Chemistry, Indiana University, 800 East Kirkwood Avenue, Bloomington, IN 47405, USA.

Abstract

The transcription factor NusG from Escherichia coli modulates the rate of transcript elongation by RNA polymerase and the efficiency of Rho-dependent transcript termination. It consists of two globular domains with an extra loop extending out of the amino-terminal domain in the position that is occupied by a third globular domain in some NusG homologues. We have tested the role of this appended mini-domain by assaying the elongation and termination enhancement activities of variants. The results show that variants with changes in their sequence do not cause a loss of functions, whereas variants with the deletions of the residues in that domain are much less active for both functions. This finding suggests that the mini-domain serves as a structural element for an interaction rather than as a site for residue-specific contacts.

PMID:: 15890417; [PubMed - indexed for MEDLINE]

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GM 56095/GM/NIGMS NIH HHS/United States

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Two structurally independent domains of E. coli NusG create regulatory plasticity via distinct interactions with RNA polymerase and regulators. [J Mol Biol. 2009]
Two structurally independent domains of E. coli NusG create regulatory plasticity via distinct interactions with RNA polymerase and regulators.
Mooney RA, Schweimer K, Rösch P, Gottesman M, Landick R. J Mol Biol. 2009 Aug 14; 391(2):341-58. Epub 2009 Jun 3.
Regulation of rho-dependent transcription termination by NusG is specific to the Escherichia coli elongation complex. [Biochemistry. 2000]
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Review Rho-dependent termination and ATPases in transcript termination.
Richardson JP. Biochim Biophys Acta. 2002 Sep 13; 1577(2):251-260.
Review Rho and RNA: models for recognition and response. [Mol Microbiol. 1994]
Review Rho and RNA: models for recognition and response.
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Cited by 2 PubMed Central articles

Functional regions of the N-terminal domain of the antiterminator RfaH. [Mol Microbiol. 2010]
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Belogurov GA, Sevostyanova A, Svetlov V, Artsimovitch I. Mol Microbiol. 2010 Apr; 76(2):286-301. Epub 2010 Feb 1.
Two structurally independent domains of E. coli NusG create regulatory plasticity via distinct interactions with RNA polymerase and regulators. [J Mol Biol. 2009]
Two structurally independent domains of E. coli NusG create regulatory plasticity via distinct interactions with RNA polymerase and regulators.
Mooney RA, Schweimer K, Rösch P, Gottesman M, Landick R. J Mol Biol. 2009 Aug 14; 391(2):341-58. Epub 2009 Jun 3.

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