Mol Cell Biol. 1992 Jun;12(6):2866-71.

A transcriptionally active DNA-binding site for human p53 protein complexes.

Funk WD, Pak DT, Karas RH, Wright WE, Shay JW.

Source

Department of Cell Biology and Neuroscience, University of Texas Southwestern Medical Center, Dallas 75235-9039.

Abstract

Recent studies have demonstrated transcriptional activation domains within the tumor suppressor protein p53, while others have described specific DNA-binding sites for p53, implying that the protein may act as a transcriptional regulatory factor. We have used a reiterative selection procedure (CASTing: cyclic amplification and selection of targets) to identify new specific binding sites for p53, using nuclear extracts from normal human fibroblasts as the source of p53 protein. The preferred consensus is the palindrome GGACATGCCCGGGCATGTCC. In vitro-translated p53 binds to this sequence only when mixed with nuclear extracts, suggesting that p53 may bind DNA after posttranslational modification or as a complex with other protein partners. When placed upstream of a reporter construct, this sequence promotes p53-dependent transcription in transient transfection assays.

PMID:: 1588974; [PubMed - indexed for MEDLINE]
PMCID: PMC364481

Free PMC Article

LinkOut - more resources

Full Text Sources

Molecular Biology Databases

Institute for Transcriptional Informatics

Supplemental Content

Related citations

YY1 and NF1 both activate the human p53 promoter by alternatively binding to a composite element, and YY1 and E1A cooperate to amplify p53 promoter activity. [Mol Cell Biol. 1996]
YY1 and NF1 both activate the human p53 promoter by alternatively binding to a composite element, and YY1 and E1A cooperate to amplify p53 promoter activity.
Furlong EE, Rein T, Martin F. Mol Cell Biol. 1996 Oct; 16(10):5933-45.
Molecular regulation of the human IL-3 gene: inducible T cell-restricted expression requires intact AP-1 and Elf-1 nuclear protein binding sites. [J Exp Med. 1993]
Molecular regulation of the human IL-3 gene: inducible T cell-restricted expression requires intact AP-1 and Elf-1 nuclear protein binding sites.
Gottschalk LR, Giannola DM, Emerson SG. J Exp Med. 1993 Nov 1; 178(5):1681-92.
Interaction of human polyomavirus BK with the tumor-suppressor protein p53. [Oncogene. 1996]
Interaction of human polyomavirus BK with the tumor-suppressor protein p53.
Shivakumar CV, Das GC. Oncogene. 1996 Jul 18; 13(2):323-32.
Review Interaction of tumor suppressor p53 with DNA and proteins. [Curr Pharm Biotechnol. 2010]
Review Interaction of tumor suppressor p53 with DNA and proteins.
Wang J, Yang J. Curr Pharm Biotechnol. 2010 Jan; 11(1):122-7.
Review Cellular and molecular advances in elucidating p53 function. [Mutat Res. 1992]
Review Cellular and molecular advances in elucidating p53 function.
Shay JW, Werbin H, Funk WD, Wright WE. Mutat Res. 1992 Aug; 277(2):163-71.

See reviews... See all...

Cited by over 100 PubMed Central articles

Tumor suppressor protein p53 recruits human Sin3B/HDAC1 complex for down-regulation of its target promoters in response to genotoxic stress. [PLoS One. 2011]
Tumor suppressor protein p53 recruits human Sin3B/HDAC1 complex for down-regulation of its target promoters in response to genotoxic stress.
Bansal N, Kadamb R, Mittal S, Vig L, Sharma R, Dwarakanath BS, Saluja D. PLoS One. 2011; 6(10):e26156. Epub 2011 Oct 20.
Early onset sebaceous carcinoma. [Diagn Pathol. 2011]
Early onset sebaceous carcinoma.
Sung D, Kaltreider SA, Gonzalez-Fernandez F. Diagn Pathol. 2011 Sep 5; 6:81. Epub 2011 Sep 5.
Genistein inhibits proliferation of colon cancer cells by attenuating a negative effect of epidermal growth factor on tumor suppressor FOXO3 activity. [BMC Cancer. 2011]
Genistein inhibits proliferation of colon cancer cells by attenuating a negative effect of epidermal growth factor on tumor suppressor FOXO3 activity.
Qi W, Weber CR, Wasland K, Savkovic SD. BMC Cancer. 2011 Jun 3; 11:219. Epub 2011 Jun 3.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Nucleotide
Primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
References for this PMC Article
Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
Free in PMC
Free full text articles in PMC
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

A transcriptionally active DNA-binding site for human p53 protein complexes.
A transcriptionally active DNA-binding site for human p53 protein complexes.
Mol Cell Biol. 1992 Jun ;12(6):2866-71.

PubMed
Viability of long term frozen cat brain in vitro.
Viability of long term frozen cat brain in vitro.
Nature. 1966 Oct 15 ;212(5059):268-70.

PubMed
Function and regulation of the beta 3 integrins in hemostasis and vascular biolo...
Function and regulation of the beta 3 integrins in hemostasis and vascular biology.
Thromb Haemost. 1995 Jul ;74(1):149-55.

PubMed
Papillary adenocarcinoma in situ of bladder.
Papillary adenocarcinoma in situ of bladder.
J Urol. 1985 Sep ;134(3):544-6.

PubMed
The functional organization of the motor system in the monkey. II. The effects o...
The functional organization of the motor system in the monkey. II. The effects of lesions of the descending brain-stem pathways.
Brain. 1968 Mar ;91(1):15-36.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Display Settings:

Send to:

A transcriptionally active DNA-binding site for human p53 protein complexes.

Source

Abstract

Publication Types, MeSH Terms, Substances, Secondary Source ID, Grant Support

Publication Types

MeSH Terms

Substances

Secondary Source ID

Grant Support

LinkOut - more resources

Full Text Sources

Molecular Biology Databases

Supplemental Content

Related citations

Cited by over 100 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information