Chemphyschem. 2005 May;6(5):956-60.

Single-molecule quantum-dot fluorescence resonance energy transfer.

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Physics Department, University of Illinois, Urbana-Champaign, Urbana, Illinois 61801, USA.

Abstract

Colloidal semiconductor quantum dots are promising for single-molecule biological imaging due to their outstanding brightness and photostability. As a proof of concept for single-molecule fluorescence resonance energy transfer (FRET) applications, we measured FRET between a single quantum dot and a single organic fluorophore Cy5. DNA Holliday junction dynamics measured with the quantum dot/Cy5 pair are identical to those obtained with the conventional Cy3/Cy5 pair, that is, conformational changes of individual molecules can be observed by using the quantum dot as the donor.

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