Display Settings:


Send to:

Choose Destination
See comment in PubMed Commons below
Science. 2005 Jun 24;308(5730):1915-20. Epub 2005 May 5.

Community proteomics of a natural microbial biofilm.

Author information

  • 1Department of Environmental Science, Policy, and Management, University of California at Berkeley, Berkeley, CA 94720, USA.


Using genomic and mass spectrometry-based proteomic methods, we evaluated gene expression, identified key activities, and examined partitioning of metabolic functions in a natural acid mine drainage (AMD) microbial biofilm community. We detected 2033 proteins from the five most abundant species in the biofilm, including 48% of the predicted proteins from the dominant biofilm organism, Leptospirillum group II. Proteins involved in protein refolding and response to oxidative stress appeared to be highly expressed, which suggests that damage to biomolecules is a key challenge for survival. We validated and estimated the relative abundance and cellular localization of 357 unique and 215 conserved novel proteins and determined that one abundant novel protein is a cytochrome central to iron oxidation and AMD formation.

[PubMed - indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Icon for HighWire Icon for Faculty of 1000
    Loading ...
    Write to the Help Desk