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Methods Enzymol. 2005;395:554-69.

Detecting differential expression of parental or progenitor alleles in genetic hybrids and allopolyploids.

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  • 1Biology Department, Washington University, Saint Louis, Missouri 63130, USA.

Abstract

Three assays useful for detecting specific RNA transcripts are primer extension, S1 nuclease protection, and reverse-transcription-cleaved amplified polymorphic sequence (RT-CAPS) analysis. All three of these techniques are used routinely for gene expression analyses and allow insights not possible by RNA blot (northern blot) hybridization. In this chapter, we describe how the primer extension, S1 nuclease protection, and RT-CAPS methods can be used to discriminate one or more parental or progenitor alleles in hybrids or allopolyploids. We discuss the rationale for using the different techniques and provide examples of the data generated.

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