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Biochem Biophys Res Commun. 2005 Jun 3;331(2):459-63.

RNA interference: a mammalian SID-1 homologue enhances siRNA uptake and gene silencing efficacy in human cells.

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  • 1Department of Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.


SID-1 is a transmembrane protein that mediates systemic RNA interference in Caenorhabditis elegans. Here we show that the mammalian SID-1 homologue FLJ20174 localizes to the cell membrane of human cells and enhances their uptake of small interfering RNA (siRNA), resulting in increased siRNA-mediated gene silencing efficacy. This is the first demonstration to show that overexpression of a membrane protein enhances siRNA internalization in mammalian cells. This observation raises the possibility of enhancing the efficacy of RNA interference.

[PubMed - indexed for MEDLINE]
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