Equine infectious anemia virus-infected dendritic cells retain antigen presentation capability

Virology. 2005 May 10;335(2):145-54. doi: 10.1016/j.virol.2005.02.013.

Abstract

To determine if equine monocyte-derived dendritic cells (DC) were susceptible to equine infectious anemia virus (EIAV) infection, ex vivo-generated DC were infected with virus in vitro. EIAV antigen was detected by immunofluorescence 3 days post-infection with maximum antigen being detected on day 4, whereas there was no antigen detected in DC incubated with the same amount of heat-inactivated EIAV. No cytolytic activity was observed after EIAV(WSU5) infection of DC. These monocyte-derived DC were more effective than macrophages and B cells in stimulating allogenic T lymphocytes. Both infected macrophages and DC stimulated similar levels of memory CTL responses in mixtures of CD8+ and CD4+ cells as detected with (51)Cr-release assays indicating that EIAV infection of DC did not alter antigen presentation. However, EIAV-infected DC were more effective than infected macrophages when used to stimulate memory CTL in isolated CD8+ cells. The maintenance of antigen processing and presenting function by EIAV-infected DC in vitro suggests that this function is maintained during in vivo infection.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigen Presentation / immunology*
  • Cells, Cultured
  • Dendritic Cells / immunology*
  • Dendritic Cells / virology*
  • Horses / immunology*
  • Horses / virology*
  • Immunologic Memory
  • Immunophenotyping
  • Infectious Anemia Virus, Equine / physiology*
  • Lymphocyte Activation
  • Lymphocyte Culture Test, Mixed
  • Macrophages / immunology
  • Macrophages / virology
  • T-Lymphocytes, Cytotoxic / immunology