Format

Send to:

Choose Destination
See comment in PubMed Commons below
J Am Chem Soc. 2005 Apr 27;127(16):5736-7.

Optically addressed droplet-based protein assay.

Author information

  • 1Molecular Physics Laboratory, SRI International, 333 Ravenswood Avenue, Menlo Park, California 94025, USA.

Abstract

We demonstrate a new method for performing protein assays with very small volumes ( approximately 1.7 muL to 14 pL). Using a laser to modify local surface energy, we manipulate, fuse, and mix droplets containing horseradish peroxidase and its substrates. A detection limit of approximately 30 attomoles of reacting enzyme was measured by optical absorption. We discuss the possibility of extending this lower limit to zeptomoles of enzyme.

PMID:
15839641
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for American Chemical Society
    Loading ...
    Write to the Help Desk