Effective protection of pigs against lethal Pseudorabies virus infection after a single injection of low-dose Sindbis-derived plasmids encoding PrV gB, gC and gD glycoproteins

Vaccine. 2005 May 16;23(26):3483-91. doi: 10.1016/j.vaccine.2004.10.050.

Abstract

This study compared protection of pigs against lethal Pseudorabies virus (PrV) infection induced by a single injection of various quantities of Sindbis virus-derived plasmids encoding PrV glycoproteins gB, gC and gD. Pigs were injected with 340, 68 or 13 microg of each plasmid. Few immune differences were observed after DNA injection and more importantly the pigs of the three groups were equally protected against virulent PrV infection. Single-shot injection of 13 microg of each PrV glycoprotein encoding Sindbis virus-derived plasmid is able to effectively protect pigs from PrV infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Genetic Vectors / genetics
  • Herpesvirus 1, Suid / drug effects*
  • Herpesvirus 1, Suid / genetics
  • Herpesvirus 1, Suid / immunology
  • Pseudorabies / immunology
  • Pseudorabies / prevention & control*
  • Pseudorabies Vaccines / administration & dosage*
  • Pseudorabies Vaccines / immunology
  • Swine Diseases / immunology
  • Swine Diseases / prevention & control*
  • Vaccines, DNA / administration & dosage*
  • Vaccines, DNA / immunology
  • Viral Envelope Proteins / genetics
  • Viral Envelope Proteins / immunology

Substances

  • Pseudorabies Vaccines
  • Vaccines, DNA
  • Viral Envelope Proteins