Molecular cloning of cDNAs encoding the GAP-associated protein p190: implications for a signaling pathway from ras to the nucleus

Cell. 1992 May 1;69(3):539-49. doi: 10.1016/0092-8674(92)90454-k.

Abstract

In mitogenically stimulated and tyrosine kinase-transformed cells, a substantial fraction of the ras GTPase-activating protein (GAP) forms a complex with a protein termed p190. We have cloned several cDNAs encoding the p190 protein. Analysis of the predicted protein sequence reveals three distinct domains with homology to previously described sequences. An N-terminal domain of p190 contains sequence motifs that are found in all of the known GTPases. At the C-terminus of the protein is a domain that contains sequences very similar to those found in the breakpoint cluster region gene product, n-chimerin, and rho GAP, all of which have been shown to possess intrinsic GAP activity on small GTPases. Finally, a 778 aa segment in the middle of p190 is nearly identical in sequence to a recently described transcriptional repressor. This raises the possibility that p190, acting via GAP, can transduce signals from p21ras to the nucleus, perhaps affecting expression of specific cellular genes.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Nucleus / physiology
  • Cells, Cultured
  • Cloning, Molecular
  • DNA / genetics
  • DNA-Binding Proteins
  • GTPase-Activating Proteins
  • Gene Expression
  • Guanine Nucleotide Exchange Factors*
  • In Vitro Techniques
  • Molecular Sequence Data
  • Molecular Weight
  • Nuclear Proteins / physiology*
  • Oligodeoxyribonucleotides / chemistry
  • Peptides / chemistry
  • Phosphoproteins / physiology*
  • Proteins / metabolism
  • Proto-Oncogene Proteins p21(ras) / physiology*
  • RNA, Messenger / genetics
  • Rats
  • Repressor Proteins / chemistry
  • Restriction Mapping
  • Sequence Alignment
  • Signal Transduction
  • ras GTPase-Activating Proteins

Substances

  • Arhgap35 protein, rat
  • DNA-Binding Proteins
  • GTPase-Activating Proteins
  • Guanine Nucleotide Exchange Factors
  • Nuclear Proteins
  • Oligodeoxyribonucleotides
  • Peptides
  • Phosphoproteins
  • Proteins
  • RNA, Messenger
  • Repressor Proteins
  • ras GTPase-Activating Proteins
  • DNA
  • Proto-Oncogene Proteins p21(ras)

Associated data

  • GENBANK/L05499
  • GENBANK/M87278
  • GENBANK/M87549
  • GENBANK/M94721
  • GENBANK/S72766
  • GENBANK/S72767
  • GENBANK/S72768
  • GENBANK/S72769
  • GENBANK/S72771
  • GENBANK/X71664