J Clin Microbiol. 2005 Apr;43(4):1995-7.

Use of single nucleotide polymorphisms in the plcR gene for specific identification of Bacillus anthracis.

Easterday WR, Van Ert MN, Simonson TS, Wagner DM, Kenefic LJ, Allender CJ, Keim P.

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Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ 86011-5640, USA.

Abstract

A TaqMan-minor groove binding assay designed around a nonsense mutation in the plcR gene was used to genotype Bacillus anthracis, B. cereus, and B. thuringiensis isolates. The assay differentiated B. anthracis from these genetic near-neighbors and determined that the nonsense mutation is ubiquitous across 89 globally and genetically diverse B. anthracis strains.

PMID:: 15815042; [PubMed - indexed for MEDLINE]
PMCID:: PMC1081367

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Use of single nucleotide polymorphisms in the plcR gene for specific identification of Bacillus anthracis.

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