Appl Environ Microbiol. 2005 Apr;71(4):2140-4.

New tool for metabolic pathway engineering in Escherichia coli: one-step method to modulate expression of chromosomal genes.

Meynial-Salles I, Cervin MA, Soucaille P.

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CRITT-Bioindustries CRT, INSA, DGBA, Toulouse, France.

Abstract

A simple and highly efficient method was developed to produce a library of Escherichia coli clones that express a particular chromosomal gene at a wide range of expression levels. The basic strategy was to replace all or part of the upstream region of a coding sequence containing the elements involved in its expression (promoter, operator, gene coding for a regulator, ribosome binding site, and start codon) with a PCR-generated library of expression cassettes.

PMID:: 15812048; [PubMed - indexed for MEDLINE]
PMCID:: PMC1082524

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Escherichia coli Proteins

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Cited by 5 PubMed Central articles

Review Application of the bacteriophage Mu-driven system for the integration/amplification of target genes in the chromosomes of engineered Gram-negative bacteria--mini review. [Appl Microbiol Biotechnol. 2011]
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Jarboe LR, Zhang X, Wang X, Moore JC, Shanmugam KT, Ingram LO. J Biomed Biotechnol. 2010; 2010:761042. Epub 2010 Apr 6.
Promoter knock-in: a novel rational method for the fine tuning of genes. [BMC Biotechnol. 2010]
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