Cardiolipin synthase of Arabidopsis thaliana

FEBS Lett. 2005 Apr 11;579(10):2161-5. doi: 10.1016/j.febslet.2005.03.007.

Abstract

Functional expression studies in microorganisms showed that the Arabidopsis thaliana gene At4g04870 represents the cardiolipin synthase (CLS) gene encoding a hydrophobic preprotein of 38 kDa with a cleavable signal peptide for the import into mitochondria. CLS of Arabidopsis over-expressed in Escherichia coli has an alkaline pH optimum, a strict requirement for divalent cations and a distinctly lower K(m) for cytidinediphosphate-diacylglycerol than for phosphatidylglycerol. It displayed a preference for both its substrates esterified with unsaturated acyl groups. Solubilization and purification experiments revealed that the protein requires a defined phospholipid environment, particularly the presence of cardiolipin, to acquire its catalytically active conformation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / enzymology*
  • Base Sequence
  • Blotting, Western
  • DNA Primers
  • Membrane Proteins / genetics*
  • Transferases (Other Substituted Phosphate Groups) / genetics*

Substances

  • DNA Primers
  • Membrane Proteins
  • Transferases (Other Substituted Phosphate Groups)
  • cardiolipin synthetase