Yeast. 1992 Jan;8(1):57-60.

Expression of recombinant platelet-derived endothelial cell growth factor in the yeast Saccharomyces cerevisiae.

Finnis C, Goodey A, Courtney M, Sleep D.

Source

Delta Biotechnology Ltd, Nottingham, U.K.

Abstract

A platelet-derived endothelial cell growth factor cDNA has been cloned, sequenced and expressed using the Saccharomyces cerevisiae PRB1 promoter. Soluble recombinant platelet-derived endothelial cell growth factor constituted 0.5-1.0% of total soluble protein. Yeast soluble protein extracts containing recombinant platelet-derived endothelial cell growth factor stimulate the growth of calf pulmonary artery endothelial cells in vitro.

PMID:: 1580101; [PubMed - indexed for MEDLINE]

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Cited by 1 PubMed Central article

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