Radiat Res. 2005 Apr;163(4):364-8.

Detection of oxidative stress induced by low- and high-linear energy transfer radiation in cultured human epithelial cells.

Wan XS, Bloch P, Ware JH, Zhou Z, Donahue JJ, Guan J, Stewart J, Kennedy AR.

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Department of Radiation Oncology, the University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA.

Abstract

A standardized dichlorofluorescin (DCF) fluorometric assay capable of measuring radiation-induced oxidative stress was used to determine the effectiveness of protons and high-mass, high-atomic number (Z) and high-energy (HZE) particles to produce oxidative stress in vitro. Protons were found to be about equally as effective as X rays in the generation of oxidative stress in cultured cells. However, 56Fe-ion beams with energies of 1 GeV/nucleon and 5 GeV/nucleon were less effective than X rays or gamma rays in inducing dichlorofluorescin (DCFH) oxidation. The relatively lower slope values for the dose responses of HZE-particle radiation-induced DCFH oxidation indicate that the sensitivity of the DCF fluorometric assay is probably dependent on the linear energy transfer (LET) of the radiation beam.

PMID:: 15799690; [PubMed - indexed for MEDLINE]

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