Synthesis of C2-chiral bifunctionalised spin labels and their application to troponin C

Shunsuke Chatani; Motoyoshi Nakamura; Hidenobu Akahane; Naoki Kohyama; Masayasu Taki; Toshiaki Arata; Yukio Yamamoto

doi:10.1039/b418016j

Synthesis of C2-chiral bifunctionalised spin labels and their application to troponin C

Chem Commun (Camb). 2005 Apr 14:(14):1880-2. doi: 10.1039/b418016j. Epub 2005 Feb 11.

Authors

Shunsuke Chatani¹, Motoyoshi Nakamura, Hidenobu Akahane, Naoki Kohyama, Masayasu Taki, Toshiaki Arata, Yukio Yamamoto

Affiliation

¹ Graduate School of Human & Environmental Studies, Kyoto University, Sakyo-ku, Yoshida, Kyoto 606-8501, Japan.

PMID: 15795774
DOI: 10.1039/b418016j

Abstract

An enantiomeric pair of C2-chiral bifunctionalised spin labels having a pyrrolidine nitroxide moiety, whose configurations were determined by X-ray crystal diffraction analysis, was prepared and applied to troponin C whose binding mode of double disulfide linkage was proved by EPR spectroscopy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Carbon / chemistry*
Chickens
Electron Spin Resonance Spectroscopy
Models, Molecular
Protein Structure, Tertiary
Spin Labels*
Stereoisomerism
Troponin C / chemical synthesis
Troponin C / chemistry*

Substances

Spin Labels
Troponin C
Carbon