Functional characterization of OXA-57, a class D beta-lactamase from Burkholderia pseudomallei

Karen E Keith; Petra C Oyston; Ben Crossett; Neil F Fairweather; Richard W Titball; Timothy R Walsh; Katherine A Brown

doi:10.1128/AAC.49.4.1639-1641.2005

Functional characterization of OXA-57, a class D beta-lactamase from Burkholderia pseudomallei

Antimicrob Agents Chemother. 2005 Apr;49(4):1639-41. doi: 10.1128/AAC.49.4.1639-1641.2005.

Authors

Karen E Keith¹, Petra C Oyston, Ben Crossett, Neil F Fairweather, Richard W Titball, Timothy R Walsh, Katherine A Brown

Affiliation

¹ Department of Biological Sciences, CMMI, Flowers Building, Imperial College London, London SW7 2AZ, United Kingdom.

Abstract

Class D beta-lactamase OXA-57 was identified in a range of isolates of Burkholderia pseudomallei and Burkholderia thailandensis. Comparative kinetic analyses of wild-type and mutant forms of B. pseudomallei OXA-57 are reported. Implications of these data for beta-lactam resistance and the proposed role of Ser-104 in beta-lactam hydrolysis are discussed.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Anti-Bacterial Agents / metabolism
Anti-Bacterial Agents / pharmacology
Burkholderia / drug effects
Burkholderia / enzymology*
Burkholderia pseudomallei / drug effects
Burkholderia pseudomallei / enzymology*
Humans
Kinetics
Molecular Sequence Data
Mutation
beta-Lactamases / chemistry
beta-Lactamases / genetics
beta-Lactamases / metabolism*
beta-Lactams / metabolism
beta-Lactams / pharmacology

Substances

Anti-Bacterial Agents
beta-Lactams
beta-Lactamases