Cloning, characterization, and expression analysis of hepcidin gene from red sea bream (Chrysophrys major)

Song-Lin Chen; Mei-Yu Xu; Xiang-Shan Ji; Guo-Cai Yu; Yang Liu

doi:10.1128/AAC.49.4.1608-1612.2005

Cloning, characterization, and expression analysis of hepcidin gene from red sea bream (Chrysophrys major)

Antimicrob Agents Chemother. 2005 Apr;49(4):1608-12. doi: 10.1128/AAC.49.4.1608-1612.2005.

Authors

Song-Lin Chen¹, Mei-Yu Xu, Xiang-Shan Ji, Guo-Cai Yu, Yang Liu

Affiliation

¹ Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Nanjing Road 106, 266071 Qingdao, People's Republic of China. chensl@ysfri.ac.cn

Abstract

A cDNA encoding hepcidin was isolated from a library of cDNA from spleen of red sea bream (Chrysophrys major) by expressed sequence tag analysis. The expression of the hepcidin mRNA in various tissues was examined. Challenge of red sea bream with Escherichia coli DH5alpha elevated hepcidin mRNA levels in spleen, gill, liver, and intestine.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Antimicrobial Cationic Peptides / chemistry*
Antimicrobial Cationic Peptides / genetics
Antimicrobial Cationic Peptides / metabolism*
Base Sequence
Cloning, Molecular*
DNA, Complementary
Hepcidins
Molecular Sequence Data
Organ Specificity
Sea Bream / genetics*
Sequence Alignment
Sequence Analysis, DNA

Substances

Antimicrobial Cationic Peptides
DNA, Complementary
Hepcidins

Associated data

GENBANK/AY452732
GENBANK/AY452733