Biophys J. 2005 May;88(5):L33-6. Epub 2005 Mar 25.

Fluctuation correlation spectroscopy with a laser-scanning microscope: exploiting the hidden time structure.

Digman MA, Sengupta P, Wiseman PW, Brown CM, Horwitz AR, Gratton E.

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Laboratory for Fluorescence Dynamics, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.

Abstract

Images obtained with a laser-scanning microscope contain a time structure that can be exploited to measure fast dynamics of molecules in solution and in cells. The spatial correlation approach provides a simple algorithm to extract this information. We describe the analysis used to process laser-scanning images of solutions and cells to obtain molecular diffusion constant in the microsecond to second timescale.

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PMCID:: PMC1305524

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