Modifying effects of Terminalia catappa on azoxymethane-induced colon carcinogenesis in male F344 rats

Eur J Cancer Prev. 2005 Apr;14(2):101-5. doi: 10.1097/00008469-200504000-00005.

Abstract

The modifying effects of dietary administration of an herb, Terminalia catappa (TC), were investigated on rat colon carcinogenesis induced by a carcinogen azoxymethane (AOM). The number of aberrant crypt foci (ACF) and beta-catenin accumulated crypts (BCACs) in the colon, and proliferating cell nuclear antigen (PCNA) labelling index in the colonic epithelium were examined in a total of 36 male F344 rats. All animals were randomly divided into five experimental groups (4-10 rats in each group). At 6 weeks of age, rats in groups 1, 2 and 3 were given s.c. injections of AOM once a week for 2 weeks at a concentration of 20 mg/kg body weight. One week before the first injection of AOM, rats in groups 2 and 3 were fed a diet containing 0.02 and 0.1% TC, respectively, throughout the experiment. Rats in group 4 were fed a diet containing 0.1% TC. Rats in group 5 were served as untreated controls. All animals were sacrificed at the experimental week 5 after the start of the experiment. Oral administration of TC at both doses significantly decreased the numbers of both ACF/colon/rat (P<0.05 for 0.02% TC, P<0.005 for 0.1% TC) and BCAC/cm/rat (P<0.05 for both 0.02 and 0.1% TC), when compared with the control group (group 1). Colonic PCNA labelling index in groups 2 and 3 was also significantly lower than that in group 1 (P<0.001 for 0.02% TC, P<0.005 for 0.1% TC). These results suggest that TC has a potent short-term chemopreventive effect on biomarkers of colon carcinogenesis and this effect may be associated with the inhibition of the development of ACF and BCACs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Oral
  • Animals
  • Azoxymethane / administration & dosage
  • Azoxymethane / toxicity
  • Biomarkers, Tumor / blood*
  • Carcinogens / administration & dosage
  • Carcinogens / toxicity
  • Cell Transformation, Neoplastic
  • Chemoprevention
  • Colonic Diseases / chemically induced
  • Colonic Diseases / prevention & control
  • Colonic Diseases / veterinary
  • Colonic Neoplasms / chemically induced
  • Colonic Neoplasms / prevention & control*
  • Colonic Neoplasms / veterinary
  • Male
  • Phytotherapy / veterinary
  • Plant Extracts / administration & dosage
  • Plant Extracts / pharmacology*
  • Proliferating Cell Nuclear Antigen / blood
  • Random Allocation
  • Rats
  • Rats, Inbred F344
  • Terminalia / chemistry*

Substances

  • Biomarkers, Tumor
  • Carcinogens
  • Plant Extracts
  • Proliferating Cell Nuclear Antigen
  • Azoxymethane