The orbits of fluid particles in two dimensions effectively act as topological obstacles to material lines. A spacetime plot of the orbits of such particles can be regarded as a braid whose properties reflect the underlying dynamics. For a chaotic flow, the braid generated by the motion of three or more fluid particles is computed. A "braiding exponent" is then defined to characterize the complexity of the braid. This exponent is proportional to the usual Lyapunov exponent of the flow, associated with separation of nearby trajectories. Measuring chaos in this manner has several advantages, especially from the experimental viewpoint, since neither nearby trajectories nor derivatives of the velocity field are needed.